{"id":92735,"date":"2024-05-13T10:15:03","date_gmt":"2024-05-13T02:15:03","guid":{"rendered":"\/\/www.dealsbon.com\/?p=92735"},"modified":"2024-05-13T10:15:09","modified_gmt":"2024-05-13T02:15:09","slug":"xhtldsyhxyzmpd-2","status":"publish","type":"post","link":"\/\/www.dealsbon.com\/blog\/92735.html","title":{"rendered":"\u4fe1\u53f7\u901a\u8def\u7684\u4e0a\u6e38\u548c\u4e0b\u6e38\u600e\u4e48\u5224\u65ad"},"content":{"rendered":"\n<p>\u3000\u3000<strong>\u4fe1\u53f7\u901a\u8def<\/strong>\u7684\u4e0a\u6e38\u548c\u4e0b\u6e38\u53ef\u4ee5\u901a\u8fc7\u591a\u79cd\u65b9\u6cd5\u6765\u5224\u65ad\u3002\u9996\u5148\uff0c\u4e0a\u6e38\u901a\u5e38\u6307\u7684\u662f\u4fe1\u53f7\u901a\u8def\u4e2d\u8d77\u59cb\u7684\u90e8\u5206\uff0c\u662f\u89e6\u53d1\u6574\u4e2a\u4fe1\u53f7\u901a\u8def\u7ea7\u8054\u53cd\u5e94\u7684\u521d\u59cb\u4e8b\u4ef6\u6216\u5206\u5b50\uff0c\u8fd9\u4e9b\u5206\u5b50\u6216\u4e8b\u4ef6\u53ef\u4ee5\u63a5\u6536\u6765\u81ea\u5916\u90e8\u73af\u5883\u7684\u523a\u6fc0\uff0c\u4ece\u800c\u542f\u52a8\u4fe1\u53f7\u4f20\u5bfc\u3002\u4e0b\u6e38\u5219\u6307\u7684\u662f\u4fe1\u53f7\u4f20\u9012\u8fc7\u7a0b\u4e2d\u4f4d\u4e8e\u4e0a\u6e38\u4e4b\u540e\u7684\u5206\u5b50\u6216\u4e8b\u4ef6\u3002<\/p>\n\n\n\n<p>\u3000\u3000\u5728\u5b9e\u9645\u64cd\u4f5c\u4e2d\uff0c\u53ef\u4ee5\u901a\u8fc7\u5bf9\u7167\u901a\u8def\u56fe\u8c31\u6765\u5224\u65ad\u4e0a\u4e0b\u6e38\uff0c\u5373\u901a\u8fc7\u6bd4\u8f83\u4fe1\u53f7\u901a\u8def\u56fe\u8c31\u4e2d\u6bcf\u4e2a\u5206\u5b50\u7684\u7279\u70b9\uff0c\u5c06\u5176\u5f52\u4e3a\u4e0a\u6e38\u6216\u4e0b\u6e38\u3002\u6b64\u5916\uff0c\u7814\u7a76\u4fe1\u53f7\u901a\u8def\u65f6\uff0c\u9274\u5b9a\u548c\u786e\u5b9a\u4fe1\u53f7\u901a\u8def\u7684\u4e0a\u6e38\u548c\u4e0b\u6e38\u5206\u5b50\u901a\u5e38\u9700\u8981\u7efc\u5408\u8fd0\u7528\u591a\u79cd\u5b9e\u9a8c\u65b9\u6cd5\u548c\u5206\u6790\u7b56\u7565\u3002<\/p>\n\n\n\n<p>\u3000\u3000\u4f8b\u5982\uff0c\u53ef\u4ee5\u901a\u8fc7\u6c89\u9ed8\u8868\u8fbe\u4e0a\u6e38\u56e0\u5b50\u6765\u89c2\u5bdf\u4e0b\u6e38\u56e0\u5b50\u7684\u8868\u8fbe\u53d8\u5316\uff0c\u5982\u679c\u4e0a\u6e38\u56e0\u5b50\u88ab\u6c89\u9ed8\u540e\uff0c\u4e0b\u6e38\u56e0\u5b50\u7684\u8868\u8fbe\u4f1a\u4e0b\u8c03\u6216\u4e0d\u8868\u8fbe\uff0c\u8fd9\u4e5f\u662f\u4e00\u79cd\u5224\u65ad\u4e0a\u4e0b\u6e38\u5173\u7cfb\u7684\u65b9\u6cd5\u3002\u53e6\u5916\uff0c\u8367\u5149\u62a5\u544a\u57fa\u56e0\u5b9e\u9a8c\u4e5f\u662f\u4e00\u79cd\u5e38\u7528\u7684\u65b9\u6cd5\uff0c\u901a\u8fc7\u5c06\u4fe1\u53f7\u901a\u8def\u7684\u4e0b\u6e38\u54cd\u5e94\u539f\u4ef6\u5e8f\u5217\u6784\u5efa\u5165\u62a5\u544a\u57fa\u56e0\u8f7d\u4f53\uff0c\u5728\u4e0d\u540c\u4e0a\u6e38\u4fe1\u53f7\u6761\u4ef6\u4e0b\uff0c\u8424\u5149\u7d20\u9176\u6d3b\u6027\u4ee3\u8868\u4e86\u901a\u8def\u7684\u4e0b\u6e38\u54cd\u5e94\u3002<\/p>\n\n\n\n<p>\u3000\u3000\u5224\u65ad\u4fe1\u53f7\u901a\u8def\u7684\u4e0a\u6e38\u548c\u4e0b\u6e38\u9700\u8981\u7efc\u5408\u8003\u8651\u5b9e\u9a8c\u6570\u636e\u3001\u901a\u8def\u56fe\u8c31\u4ee5\u53ca\u5177\u4f53\u7684\u751f\u7269\u5b66\u80cc\u666f\uff0c\u901a\u8fc7\u591a\u79cd\u65b9\u6cd5\u548c\u6280\u672f\u624b\u6bb5\u8fdb\u884c\u7efc\u5408\u5206\u6790\u548c\u5224\u65ad\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u3000\u3000\u4e00\u3001 \u4fe1\u53f7\u901a\u8def\u56fe\u8c31\u7684\u6784\u5efa\u548c\u89e3\u8bfb\u65b9\u6cd5\u6709\u54ea\u4e9b?<\/h2>\n\n\n\n<p>\u3000\u3000\u4fe1\u53f7\u901a\u8def\u56fe\u8c31\u7684\u6784\u5efa\u548c\u89e3\u8bfb\u65b9\u6cd5\u4e3b\u8981\u5305\u62ec\u4ee5\u4e0b\u51e0\u4e2a\u65b9\u9762\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u3000\u3000<strong>\u4f7f\u7528KEGGMapper\u8fdb\u884c\u6570\u636e\u5206\u6790<\/strong>\uff1a\u9996\u5148\uff0c\u9700\u8981\u901a\u8fc7KEGGMapper\u641c\u7d22\u7ed3\u679c\u6765\u6574\u7406\u4e00\u4e2a\u8868\u683c\uff0c\u6536\u96c65-10\u4e2a\u5206\u5b50\u3002\u8fd9\u4e00\u6b65\u9aa4\u53ef\u4ee5\u5e2e\u52a9\u7814\u7a76\u8005\u5feb\u901f\u627e\u5230\u4e0e\u4ed6\u4eec\u7814\u7a76\u4e3b\u9898\u76f8\u5173\u7684\u4fe1\u53f7\u901a\u8def\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u5229\u7528GO\u548cKEGG\u8fdb\u884c\u5bcc\u96c6\u5206\u6790<\/strong>\uff1a\u901a\u8fc7GO\u548cKEGG\u8fdb\u884c\u5bcc\u96c6\u5206\u6790\uff0c\u53ef\u4ee5\u5e2e\u52a9\u7814\u7a76\u8005\u7406\u89e3\u7279\u5b9a\u6307\u6807\u7684\u542b\u4e49\u4ee5\u53ca\u5982\u4f55\u4f7f\u7528\u8fd9\u4e9b\u6307\u6807\u8fdb\u884c\u7ed3\u679c\u89e3\u8bfb\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u4f7f\u7528Pathway Builder Tool\u8f6f\u4ef6<\/strong>\uff1a\u8fd9\u6b3e\u8f6f\u4ef6\u5141\u8bb8\u7528\u6237\u9009\u62e9\u6700\u9002\u5408\u7684\u901a\u8def\u6a21\u677f\uff0c\u5e76\u5728\u9002\u5408\u7684\u901a\u8def\u6a21\u677f\u4e0b\u8fdb\u884c\u64cd\u4f5c\u3002\u8fd9\u5bf9\u4e8e\u90a3\u4e9b\u5e0c\u671b\u5728\u8f6f\u4ef6\u4e2d\u7ed8\u5236\u6216\u67e5\u770b\u4fe1\u53f7\u901a\u8def\u56fe\u7684\u4eba\u6765\u8bf4\u662f\u4e00\u4e2a\u5f88\u597d\u7684\u5de5\u5177\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u53c2\u8003\u4e13\u4e1a\u8f6f\u4ef6\u5982IPA(Ingenuity Pathway Analysis)<\/strong>\uff1a\u8fd9\u662f\u4e00\u6b3e\u5546\u4e1a\u6027\u901a\u8def\u5206\u6790\u8f6f\u4ef6\uff0c\u4e13\u4e3a\u533b\u5b66\u670b\u53cb\u8bbe\u8ba1\uff0c\u4e0d\u9700\u8981\u638c\u63e1\u592a\u590d\u6742\u7684\u529f\u80fd\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u5b66\u4e60\u5982\u4f55\u7ed8\u5236\u4fe1\u53f7\u901a\u8def\u56fe<\/strong>\uff1a\u53ef\u4ee5\u5c1d\u8bd5\u4f7f\u7528pathway builder tool\u8f6f\u4ef6\uff0c\u8fd9\u6b3e\u8f6f\u4ef6\u7b80\u5355\u6613\u5b66\uff0c\u5373\u4fbf\u662f\u96f6\u57fa\u7840\u4e5f\u80fd\u8f7b\u677e\u4e0a\u624b\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u63a2\u7d22\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u6280\u672f\u53ca\u5176\u5728\u4eba\u7c7b\u7ec6\u80de\u56fe\u8c31\u6784\u5efa\u4e2d\u7684\u5e94\u7528<\/strong>\uff1a\u8fd9\u6d89\u53ca\u5230\u5728\u7279\u5b9a\u65f6\u95f4\u70b9(\u5982\u80da\u80ce\u671f\u7b2c6.5\u5929)\u6536\u96c6\u65e9\u671f\u539f\u80a0\u80da\u5f62\u6210\u65f6\u671f\u7684\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7279\u5f81\u4ee5\u53ca\u8f6c\u5f55\u56e0\u5b50\u548c\u4fe1\u53f7\u901a\u8def\u8c03\u63a7\u7f51\u7edc\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u3000\u3000\u6784\u5efa\u548c\u89e3\u8bfb\u4fe1\u53f7\u901a\u8def\u56fe\u8c31\u7684\u65b9\u6cd5\u6d89\u53ca\u5230\u4f7f\u7528\u7279\u5b9a\u7684\u6570\u636e\u5e93\u548c\u5de5\u5177\uff0c\u5982KEGGMapper\u3001GO\u3001KEGG\u5bcc\u96c6\u5206\u6790\uff0c\u4ee5\u53ca\u5229\u7528\u4e13\u4e1a\u8f6f\u4ef6\u5982Pathway Builder Tool\u8fdb\u884c\u6570\u636e\u5206\u6790\u548c\u7ed3\u679c\u89e3\u8bfb\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u3000\u3000\u4e8c\u3001 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class=\"wp-block-heading\">\u3000\u3000\u4e09\u3001 \u8367\u5149\u62a5\u544a\u57fa\u56e0\u5b9e\u9a8c\u5728\u4fe1\u53f7\u901a\u8def\u7814\u7a76\u4e2d\u7684\u5e94\u7528\u548c\u6b65\u9aa4\u662f\u4ec0\u4e48?<\/h2>\n\n\n\n<p>\u3000\u3000\u8367\u5149\u62a5\u544a\u57fa\u56e0\u5b9e\u9a8c\u5728\u4fe1\u53f7\u901a\u8def\u7814\u7a76\u4e2d\u7684\u5e94\u7528\u548c\u6b65\u9aa4\u4e3b\u8981\u6d89\u53ca\u4ee5\u4e0b\u51e0\u4e2a\u65b9\u9762\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u3000\u3000<strong>\u5b9e\u9a8c\u76ee\u7684\u7684\u8bbe\u5b9a<\/strong>\uff1a\u9996\u5148\u9700\u8981\u660e\u786e\u5b9e\u9a8c\u7684\u76ee\u7684\u662f\u4ec0\u4e48\uff0c\u8fd9\u901a\u5e38\u662f\u4e3a\u4e86\u9a8c\u8bc1\u67d0\u79cd\u751f\u7269\u5b66\u8fc7\u7a0b\u6216\u673a\u5236\u662f\u5426\u53d7\u5230\u7279\u5b9a\u57fa\u56e0\u6216\u86cb\u767d\u8d28\u7684\u8c03\u63a7\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u6784\u5efa\u62a5\u544a\u57fa\u56e0\u8d28\u7c92<\/strong>\uff1a\u5c06\u76ee\u7684\u7247\u6bb5\u63d2\u5165\u5230\u8367\u5149\u7d20\u9176\u8868\u8fbe\u7684\u62a5\u544a\u57fa\u56e0\u8f7d\u4f53\u4e0a\uff0c\u5982pGL3-basic\uff0c\u4ee5\u6784\u5efa\u6210\u62a5\u544a\u57fa\u56e0\u8d28\u7c92\uff0c\u4f7f\u8fd9\u6bb5\u5e8f\u5217\u8c03\u63a7luciferase\u7684\u8f6c\u5f55\u8868\u8fbe\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u8f6c\u67d3\u7ec6\u80de<\/strong>\uff1a\u5c06\u6784\u5efa\u597d\u7684\u62a5\u544a\u57fa\u56e0\u8d28\u7c92\u8f6c\u67d3\u81f3\u7ec6\u80de\u5185\u8fdb\u884c\u8868\u8fbe\uff0c\u901a\u5e38\u662f\u572824-well\u57f9\u517b\u677f\u4e2d\u8fdb\u884c\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u88c2\u89e3\u6db2\u88c2\u89e3\u7ec6\u80de<\/strong>\uff1a\u8fd9\u4e00\u6b65\u9aa4\u53ef\u80fd\u6d89\u53ca\u5230\u4f7f\u7528\u88c2\u89e3\u6db2\u6765\u5904\u7406\u7ec6\u80de\u6837\u672c\uff0c\u4ee5\u4fbf\u4e8e\u540e\u7eed\u7684\u8367\u5149\u68c0\u6d4b\u548c\u6570\u636e\u5206\u6790\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u8367\u5149\u68c0\u6d4b<\/strong>\uff1a\u4f7f\u7528\u8367\u5149\u7d20\u9176(\u5982\u706b\u866b\u8367\u5149\u7d20\u9176\u548c\u6d77\u80be\u8367\u5149\u7d20\u9176)\u4f5c\u4e3a\u5e95\u7269\u6765\u68c0\u6d4b\u5176\u6d3b\u6027\uff0c\u4ee5\u53ca\u901a\u8fc7\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u7b49\u65b9\u6cd5\u6765\u9a8c\u8bc1\u4e0a\u6e38\u523a\u6fc0\u5bf9\u4e0b\u6e38\u9776\u57fa\u56e0mRNA\u8868\u8fbe\u6c34\u5e73\u7684\u5f71\u54cd\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u6570\u636e\u5206\u6790<\/strong>\uff1a\u6700\u540e\u4e00\u6b65\u662f\u5bf9\u6536\u96c6\u5230\u7684\u6570\u636e\u8fdb\u884c\u5206\u6790\uff0c\u4ee5\u5f97\u51fa\u5b9e\u9a8c\u7ed3\u8bba\u3002\u8fd9\u5305\u62ec\u4f46\u4e0d\u9650\u4e8e\u9a8c\u8bc1miRNA\u4e0e3'UTR\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u6216\u8005\u9a8c\u8bc1\u9798\u78f7\u8102\u4ee3\u8c22\u4e2d\u5173\u952e\u9176A\u7684\u6d3b\u6027\u662f\u5426\u53d7\u5173\u6ce8\u57fa\u56e0X\u7684\u5f71\u54cd\u3002<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">\u3000\u3000\u56db\u3001 \u5728\u4fe1\u53f7\u901a\u8def\u7684\u4e0a\u4e0b\u6e38\u9274\u5b9a\u4e2d\uff0c\u54ea\u4e9b\u5b9e\u9a8c\u6280\u672f\u6700\u6709\u6548?<\/h2>\n\n\n\n<p>\u3000\u3000\u5728\u4fe1\u53f7\u901a\u8def\u7684\u4e0a\u4e0b\u6e38\u9274\u5b9a\u4e2d\uff0c\u6700\u6709\u6548\u7684\u5b9e\u9a8c\u6280\u672f\u5305\u62ec\u514d\u75ab\u5171\u6c89\u6dc0\u3001\u86cb\u767d\u8d28\u8c31\u6280\u672f(MS)\u3001\u65e0\u6807\u7b7eLC-MS\/MS\u86cb\u767d\u7ec4\u5b66\u65b9\u6cd5\u4ee5\u53ca\u62a5\u544a\u57fa\u56e0\u68c0\u6d4b(Reporter gene assays)\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u3000\u3000<strong>\u514d\u75ab\u5171\u6c89\u6dc0<\/strong>\uff1a\u8fd9\u662f\u4e00\u79cd\u5e38\u7528\u7684\u6280\u672f\uff0c\u7528\u4e8e\u9274\u5b9a\u86cb\u767d\u8d28\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u5c39\u822a\u8bfe\u9898\u7ec4\u901a\u8fc7\u514d\u75ab\u5171\u6c89\u6dc0\u6280\u672f\u53d1\u73b0USP15\u662fcGAS\u7684\u4e92\u4f5c\u86cb\u767d\uff0c\u738b\u5174\u526f\u6559\u6388\u56e2\u961f\u4e5f\u901a\u8fc7\u514d\u75ab\u5171\u6c89\u6dc0\u7ed3\u5408\u8d28\u8c31\u9274\u5b9a\u53d1\u73b0\u65b0\u7684Fat\u4fe1\u53f7\u54cd\u5e94\u86cb\u767dDlish\u4e0e\u5df2\u77e5\u7684Hippo\u4fe1\u53f7\u901a\u8def\u4e0a\u6e38\u8c03\u63a7\u86cb\u767dExpanded\u53d1\u751f\u76f8\u4e92\u4f5c\u7528\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u86cb\u767d\u8d28\u8c31\u6280\u672f(MS)<\/strong>\uff1a\u8fd9\u662f\u5f53\u524d\u9274\u5b9a\u86cb\u767d\u6700\u5e38\u7528\u4e14\u6709\u6548\u7684\u65b9\u6cd5\u4e4b\u4e00\u3002\u5b83\u53ef\u4ee5\u7528\u4e8e\u9274\u5b9a\u7ec6\u80de\u4e2d\u7684\u86cb\u767d\u8d28\u5e76\u63ed\u793a\u4fe1\u53f7\u901a\u8def\u8c03\u63a7\u7684\u53d8\u5316\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u65e0\u6807\u7b7eLC-MS\/MS\u86cb\u767d\u7ec4\u5b66\u65b9\u6cd5<\/strong>\uff1a\u8fd9\u79cd\u65b9\u6cd5\u88ab\u7528\u4e8e\u4ece\u7279\u5b9a\u7684\u7ec6\u80de\u7cfb\u4e2d\u9274\u5b9a\u5dee\u5f02\u6027\u7684\u86cb\u767d\u548c\u4fe1\u53f7\u901a\u8def\u3002\u8fd9\u79cd\u6280\u672f\u80fd\u591f\u63d0\u4f9b\u5173\u4e8e\u7ec6\u80de\u72b6\u6001\u53d8\u5316\u7684\u8be6\u7ec6\u4fe1\u606f\uff0c\u6709\u52a9\u4e8e\u7406\u89e3\u4fe1\u53f7\u901a\u8def\u7684\u8c03\u63a7\u673a\u5236\u3002<\/li>\n\n\n\n<li>\u3000\u3000<strong>\u62a5\u544a\u57fa\u56e0\u68c0\u6d4b(Reporter gene assays)<\/strong>\uff1a\u8fd9\u662f\u4e00\u79cd\u5229\u7528\u8f6c\u5f55\u62a5\u544a\u7cfb\u7edf\u6765\u68c0\u6d4b\u7279\u5b9a\u4fe1\u53f7\u901a\u8def\u6d3b\u6027\u7684\u6280\u672f\u3002\u901a\u8fc7\u8fd9\u79cd\u65b9\u6cd5\uff0c\u53ef\u4ee5\u76f4\u89c2\u5730\u8bc4\u4f30\u7279\u5b9a\u4fe1\u53f7\u901a\u8def\u662f\u5426\u88ab\u6fc0\u6d3b\uff0c\u4ece\u800c\u4e3a\u7814\u7a76\u4fe1\u53f7\u901a\u8def\u63d0\u4f9b\u4e86\u4e00\u79cd\u5feb\u901f\u6709\u6548\u7684\u624b\u6bb5\u3002<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">\u3000\u3000\u4e94\u3001 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